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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 9, Iss. 3 — Mar. 6, 2014

Lensless phase contrast microscopy based on multiwavelength Fresnel diffraction

Daniel W. E. Noom, Kjeld S. E. Eikema, and Stefan Witte  »View Author Affiliations

Optics Letters, Vol. 39, Issue 2, pp. 193-196 (2014)

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We demonstrate a compact, wide-field, quantitative phase contrast microscope that does not require lenses for image formation. High-resolution images are retrieved from Fresnel diffraction patterns recorded at multiple wavelengths, combined with a robust iterative phase retrieval algorithm. Quantitative phase contrast images of living cultured neurons are obtained with a transverse resolution of <2μm. Our system is well suited for high-resolution live cell imaging and provides a compact, cost-effective alternative to full-sized phase-contrast microscopes.

© 2014 Optical Society of America

OCIS Codes
(100.5070) Image processing : Phase retrieval
(110.1650) Imaging systems : Coherence imaging
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.0180) Microscopy : Microscopy

ToC Category:

Original Manuscript: October 29, 2013
Manuscript Accepted: November 15, 2013
Published: January 3, 2014

Virtual Issues
Vol. 9, Iss. 3 Virtual Journal for Biomedical Optics

Daniel W. E. Noom, Kjeld S. E. Eikema, and Stefan Witte, "Lensless phase contrast microscopy based on multiwavelength Fresnel diffraction," Opt. Lett. 39, 193-196 (2014)

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