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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 9, Iss. 4 — Apr. 1, 2014

Confocal supercritical angle microscopy for cell membrane imaging

Siddharth Sivankutty, Thomas Barroca, Céline Mayet, Guillaume Dupuis, Emmanuel Fort, and Sandrine Lévêque-Fort  »View Author Affiliations


Optics Letters, Vol. 39, Issue 3, pp. 555-558 (2014)
http://dx.doi.org/10.1364/OL.39.000555


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Abstract

We demonstrate subwavelength sectioning on biological samples with a conventional confocal microscope. This optical sectioning is achieved by the phenomenon of supercritical angle fluorescence, wherein only a fluorophore next to the interface of a refractive index discontinuity can emit propagating components of radiation into the so-called forbidden angles. The simplicity of this technique allows it to be integrated with a high numerical aperture confocal scanning microscope by only a simple modification on the detection channel. Confocal-supercritical angular fluorescence microscopy would be a powerful tool to achieve high-resolution surface imaging, especially for membrane imaging in biological samples.

© 2014 Optical Society of America

OCIS Codes
(180.1790) Microscopy : Confocal microscopy
(240.0240) Optics at surfaces : Optics at surfaces
(260.6970) Physical optics : Total internal reflection

ToC Category:
Microscopy

History
Original Manuscript: November 11, 2013
Revised Manuscript: December 19, 2013
Manuscript Accepted: December 19, 2013
Published: January 24, 2014

Virtual Issues
Vol. 9, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Siddharth Sivankutty, Thomas Barroca, Céline Mayet, Guillaume Dupuis, Emmanuel Fort, and Sandrine Lévêque-Fort, "Confocal supercritical angle microscopy for cell membrane imaging," Opt. Lett. 39, 555-558 (2014)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-39-3-555

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