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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 9, Iss. 4 — Apr. 1, 2014

View synthesis with a partitioned-aperture microscope

J.-C. Baritaux, C. R. Chan, J. Li, and J. Mertz  »View Author Affiliations

Optics Letters, Vol. 39, Issue 3, pp. 685-688 (2014)

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We present a simple and fast algorithm for view synthesis based on the acquisition of four high-resolution oblique images with a conventional widefield microscope. The images are acquired simultaneously using a partitioned aperture add-on. The technique provides physically valid views of thin samples that are transmitting or fluorescent, as demonstrated with biopsied tissue or green fluorescent protein-labeled brain slices. The goal of this technique is to facilitate image interpretation by conferring impressions of depth that are otherwise absent in standard microscope images.

© 2014 Optical Society of America

OCIS Codes
(100.2980) Image processing : Image enhancement
(100.3010) Image processing : Image reconstruction techniques
(180.0180) Microscopy : Microscopy
(110.1758) Imaging systems : Computational imaging

ToC Category:
Imaging Systems

Original Manuscript: November 5, 2013
Revised Manuscript: December 9, 2013
Manuscript Accepted: December 19, 2013
Published: January 30, 2014

Virtual Issues
Vol. 9, Iss. 4 Virtual Journal for Biomedical Optics

J.-C. Baritaux, C. R. Chan, J. Li, and J. Mertz, "View synthesis with a partitioned-aperture microscope," Opt. Lett. 39, 685-688 (2014)

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