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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 9, Iss. 4 — Apr. 1, 2014

Structured illumination diffraction phase microscopy for broadband, subdiffraction resolution, quantitative phase imaging

Shwetadwip Chowdhury and Joseph Izatt  »View Author Affiliations


Optics Letters, Vol. 39, Issue 4, pp. 1015-1018 (2014)
http://dx.doi.org/10.1364/OL.39.001015


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Abstract

Structured illumination microscopy (SIM) is an established technique that allows subdiffraction resolution imaging by heterodyning high sample frequencies into the system’s passband via structured illumination. However, until now, SIM has been typically used to achieve subdiffraction resolution for intensity-based imaging. Here, we present a novel optical setup that uses structured illumination with a broadband light source to obtain noise-reduced, subdiffraction resolution, quantitative phase imaging (QPM) of cells. We compare this with a previous work for subdiffraction QPM imaging via SIM that used a laser source, and was thus still corrupted by coherent noise.

© 2014 Optical Society of America

OCIS Codes
(030.0030) Coherence and statistical optics : Coherence and statistical optics
(090.0090) Holography : Holography
(100.6640) Image processing : Superresolution
(180.0180) Microscopy : Microscopy

ToC Category:
Imaging Systems

History
Original Manuscript: October 23, 2013
Revised Manuscript: January 12, 2014
Manuscript Accepted: January 13, 2014
Published: February 12, 2014

Virtual Issues
Vol. 9, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Shwetadwip Chowdhury and Joseph Izatt, "Structured illumination diffraction phase microscopy for broadband, subdiffraction resolution, quantitative phase imaging," Opt. Lett. 39, 1015-1018 (2014)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-39-4-1015


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