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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 9, Iss. 5 — Apr. 29, 2014

Refocusing based on amplitude analysis in color digital holographic microscopy

Jérôme Dohet-Eraly, Catherine Yourassowsky, and Frank Dubois  »View Author Affiliations

Optics Letters, Vol. 39, Issue 5, pp. 1109-1112 (2014)

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A refocusing criterion adapted to red–green–blue (RGB) digital holographic microscopy is established. It is applicable for both amplitude and phase objects. This color criterion is based on a monochromatic criterion, using the integrated modulus amplitude. Simulated RGB holograms show the value of having color information, even for colorless samples; in addition, the position of the focus plane along the optical axis is determined more accurately. Simulations take into account both the numerical apertures of lenses and noise during the holographic process. We also implement an algorithm exponentially reducing the computation time required for detecting the focus plane. The method is validated on experimental holograms.

© 2014 Optical Society of America

OCIS Codes
(090.2880) Holography : Holographic interferometry
(110.0180) Imaging systems : Microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(090.1705) Holography : Color holography
(090.1995) Holography : Digital holography

ToC Category:

Original Manuscript: December 10, 2013
Revised Manuscript: January 14, 2014
Manuscript Accepted: January 14, 2014
Published: February 17, 2014

Virtual Issues
Vol. 9, Iss. 5 Virtual Journal for Biomedical Optics

Jérôme Dohet-Eraly, Catherine Yourassowsky, and Frank Dubois, "Refocusing based on amplitude analysis in color digital holographic microscopy," Opt. Lett. 39, 1109-1112 (2014)

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