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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 9, Iss. 5 — Apr. 29, 2014

Schlieren confocal microscopy for phase-relief imaging

Hao Xie, Dayong Jin, Junjie Yu, Tong Peng, Yichen Ding, Changhe Zhou, and Peng Xi  »View Author Affiliations


Optics Letters, Vol. 39, Issue 5, pp. 1238-1241 (2014)
http://dx.doi.org/10.1364/OL.39.001238


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Abstract

We demonstrate a simple phase-sensitive microscopic technique capable of imaging the phase gradient of a transparent specimen, based on the Schlieren modulation and confocal laser scanning microscopy (CLSM). The incident laser is refracted by the phase gradient of the specimen and excites a fluorescence plate behind the specimen to create a secondary illumination; then the fluoresence is modulated by a partial obstructor before entering the confocal pinhole. The quantitative relationship between the image intensity and the sample phase gradient can be derived. This setup is very easy to be adapted to current confocal setups, so that multimodality fluorescence/structure images can be obtained within a single system.

© 2014 Optical Society of America

OCIS Codes
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.5810) Microscopy : Scanning microscopy

ToC Category:
Microscopy

History
Original Manuscript: October 10, 2013
Revised Manuscript: December 9, 2013
Manuscript Accepted: January 13, 2014
Published: February 25, 2014

Virtual Issues
Vol. 9, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Hao Xie, Dayong Jin, Junjie Yu, Tong Peng, Yichen Ding, Changhe Zhou, and Peng Xi, "Schlieren confocal microscopy for phase-relief imaging," Opt. Lett. 39, 1238-1241 (2014)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-39-5-1238

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