We demonstrate 3D differential phase-contrast (DPC) microscopy, based on computational illumination with a programmable LED array. By capturing intensity images with various illumination angles generated by sequentially patterning an LED array source, we digitally refocus images through various depths via light field processing. The intensity differences from images taken at complementary illumination angles are then used to generate DPC images, which are related to the gradient of phase. The proposed method achieves 3D DPC with simple, inexpensive optics and no moving parts. We experimentally demonstrate our method by imaging a camel hair sample in 3D.
© 2014 Optical Society of America
Original Manuscript: December 3, 2013
Revised Manuscript: January 22, 2014
Manuscript Accepted: January 27, 2014
Published: February 28, 2014
Vol. 9, Iss. 5 Virtual Journal for Biomedical Optics
Lei Tian, Jingyan Wang, and Laura Waller, "3D differential phase-contrast microscopy with computational illumination using an LED array," Opt. Lett. 39, 1326-1329 (2014)