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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 9, Iss. 5 — Apr. 29, 2014

Analytic method to optimize aperture design in focal modulation microscopy

Yubo Duan, Colin J. R. Sheppard, Shakil Rehman, and Nanguang Chen  »View Author Affiliations


Optics Letters, Vol. 39, Issue 6, pp. 1677-1680 (2014)
http://dx.doi.org/10.1364/OL.39.001677


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Abstract

Focal modulation microscopy (FMM) has been demonstrated more effective than confocal microscopy for imaging of thick biological tissues. To improve its penetration depth further, we propose a simple analytical method to enlarge the modulation depth, the unique property of FMM directly linked to its signal-to-noise ratio. The modulation depth increases as the excitation intensity of the binary phase aperture status is pushed further away from the focal region of the detection optics, thereby creating a dark region in the focal volume, which we call maximally flat crater (MFC). By direct algebraic manipulation, MFCs are achieved for both scalar and vector diffraction optics. Numerical results show that the modulation depth from MFC is very close to the maximum values, with a small difference less than 3% for the same number of subapertures. Applications of bifocus produced by MFC apertures are also discussed.

© 2014 Optical Society of America

OCIS Codes
(050.1220) Diffraction and gratings : Apertures
(050.1940) Diffraction and gratings : Diffraction
(170.1630) Medical optics and biotechnology : Coded aperture imaging
(180.1790) Microscopy : Confocal microscopy
(180.5810) Microscopy : Scanning microscopy
(220.1230) Optical design and fabrication : Apodization

ToC Category:
Microscopy

History
Original Manuscript: January 3, 2014
Manuscript Accepted: February 14, 2014
Published: March 14, 2014

Virtual Issues
Vol. 9, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Yubo Duan, Colin J. R. Sheppard, Shakil Rehman, and Nanguang Chen, "Analytic method to optimize aperture design in focal modulation microscopy," Opt. Lett. 39, 1677-1680 (2014)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=ol-39-6-1677


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