Abstract

We propose here a new three-dimensional (3D) holographic tracking method capable to track, simultaneously and in a single step, all the spatial coordinates of micro-objects. The approach is based on the enhanced correlation coefficient (ECC) maximization method but applied, for the first time to the best of our knowledge, directly on the holographic reconstructed complex wave fields. The key novelty of the proposed strategy is its ability to calculate simultaneously the 3D coordinates of cells, without decoupling the contribution of amplitude and phase. The proposed strategy is tested on living cells (i.e., NIH 3T3 mouse fibroblast) flowing into a microfluidic channel and compared with classical holographic tracking approach. Theoretical description and experimental validation of the proposed strategy are reported.

© 2014 Optical Society of America

Recent advances in holographic 3D particle tracking

Pasquale Memmolo, Lisa Miccio, Melania Paturzo, Giuseppe Di Caprio, Giuseppe Coppola, Paolo A. Netti, and Pietro Ferraro
Adv. Opt. Photon. 7(4) 713-755 (2015)

Simple and fast spectral domain algorithm for quantitative phase imaging of living cells with digital holographic microscopy

Junwei Min, Baoli Yao, Steffi Ketelhut, Christian Engwer, Burkhard Greve, and Björn Kemper
Opt. Lett. 42(2) 227-230 (2017)

On the holographic 3D tracking of in vitro cells characterized by a highly-morphological change

Pasquale Memmolo, Maria Iannone, Maurizio Ventre, Paolo Antonio Netti, Andrea Finizio, Melania Paturzo, and Pietro Ferraro
Opt. Express 20(27) 28485-28493 (2012)

Holographic time-resolved particle tracking by means of three-dimensional volumetric deconvolution

Tatiana Latychevskaia and Hans-Werner Fink
Opt. Express 22(17) 20994-21003 (2014)

Accurate position tracking of optically trapped live cells

Niall McAlinden, David G. Glass, Owain R. Millington, and Amanda J. Wright
Biomed. Opt. Express 5(4) 1026-1037 (2014)