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Non-Scanning Multimode Beam Led to Optogenetic Stimulation

Kamal Dhakal, Ling Gu, Bryan J. Black, and Samarendra K. Mohanty

Open Access Open Access

Abstract

Functional connections between billions of neurons in central and peripheral nervous systems enable thousands of specialized functions, from sensory to motor activities. This has created significant priorities in the scientific community to develop precise stimulation and imaging tools for probing complex neural circuitry. We demonstrated a two-photon optogenetic stimulation (TPOS) of excitable cells and brain slices using scanning near-infrared (NIR) femtosecond laser beams five years ago. Since then, applications of this method for probing neural circuitry have been growing.

© 2013 Optical Society of America

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