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Applied Optics

Applied Optics


  • Editor: Joseph N. Mait
  • Vol. 52, Iss. 1 — Jan. 1, 2013
  • pp: A92–A96

Gradient field microscopy for label-free diagnosis of human biopsies [Invited]

Taewoo Kim, Shamira Sridharan, André Kajdacsy-Balla, Krishnarao Tangella, and Gabriel Popescu  »View Author Affiliations

Applied Optics, Vol. 52, Issue 1, pp. A92-A96 (2013)

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We introduce a label-free method for detecting high-grade prostatic intraepithelial neoplasia (HGPIN) in unstained biopsies. We image this condition based on the identification of basal cells in biopsies that otherwise would resemble prostate cancer by unassisted histologic examination. Gradient field microscopy (GFM) is used as a label-free imaging method which increases the contrast of a transparent sample by taking the first-order phase derivative that is very sensitive to rapid refractive index changes. GFM is able to image the basal cell layers in HGPIN biopsies because of their rapid refractive index changes at the periphery of small glandular structures.

© 2012 Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.1650) Medical optics and biotechnology : Coherence imaging
(170.4730) Medical optics and biotechnology : Optical pathology
(170.6935) Medical optics and biotechnology : Tissue characterization

Original Manuscript: August 16, 2012
Revised Manuscript: September 26, 2012
Manuscript Accepted: September 30, 2012
Published: October 30, 2012

Virtual Issues
Vol. 8, Iss. 2 Virtual Journal for Biomedical Optics

Taewoo Kim, Shamira Sridharan, André Kajdacsy-Balla, Krishnarao Tangella, and Gabriel Popescu, "Gradient field microscopy for label-free diagnosis of human biopsies [Invited]," Appl. Opt. 52, A92-A96 (2013)

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