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Optics Letters

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  • Editor: Alan E. Willner
  • Vol. 37, Iss. 5 — Mar. 1, 2012
  • pp: 948–950

Extraction of intrinsic fluorescence from single fiber fluorescence measurements on a turbid medium

Stephen C. Kanick, Dominic J. Robinson, Henricus J. C. M. Sterenborg, and Arjen Amelink  »View Author Affiliations


Optics Letters, Vol. 37, Issue 5, pp. 948-950 (2012)
http://dx.doi.org/10.1364/OL.37.000948


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Abstract

This study utilizes Monte Carlo simulations of single fiber fluorescence to develop an empirical model that corrects for the influence of scattering and absorption on fluorescence intensity (FSF). The model expresses FSF in terms of the reduced scattering coefficient (μs) and absorption coefficient (μa), each determined independently at excitation and emission wavelengths (λx and λm), and the fiber diameter (df). This model returns accurate descriptions (mean residual <6%) of FSF across a biologically relevant range of μs and μa values and is insensitive to the form of the scattering phase function.

© 2012 Optical Society of America

OCIS Codes
(060.2310) Fiber optics and optical communications : Fiber optics
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(290.7050) Scattering : Turbid media
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence

ToC Category:
Fiber Optics and Optical Communications

History
Original Manuscript: November 21, 2011
Revised Manuscript: January 24, 2012
Manuscript Accepted: January 25, 2012
Published: February 28, 2012

Virtual Issues
Vol. 7, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Stephen C. Kanick, Dominic J. Robinson, Henricus J. C. M. Sterenborg, and Arjen Amelink, "Extraction of intrinsic fluorescence from single fiber fluorescence measurements on a turbid medium," Opt. Lett. 37, 948-950 (2012)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-37-5-948


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