OSA's Digital Library

Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 38, Iss. 10 — May. 15, 2013
  • pp: 1697–1699

Fast flexible multiphoton fluorescence lifetime imaging using acousto-optic deflector

Jing Qi, Yonghong Shao, Lixin Liu, Kaige Wang, Tongsheng Chen, Junle Qu, and Hanben Niu  »View Author Affiliations

Optics Letters, Vol. 38, Issue 10, pp. 1697-1699 (2013)

View Full Text Article

Enhanced HTML    Acrobat PDF (304 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We present a fast and flexible fluorescence lifetime imaging microscopy which uses a two-dimensional acousto-optic deflector to achieve fast beam scanning across the sample and provides random access to the regions of interests (ROI). Experimental results using standard fluorescent dye and biological samples show that this system can make addressable fluorescence lifetime measurements and perform fast and flexible fluorescence lifetime imaging particularly to the discontinuous ROI in the sample.

© 2013 Optical Society of America

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:

Original Manuscript: February 11, 2013
Revised Manuscript: April 5, 2013
Manuscript Accepted: April 5, 2013
Published: May 13, 2013

Virtual Issues
Vol. 8, Iss. 6 Virtual Journal for Biomedical Optics

Jing Qi, Yonghong Shao, Lixin Liu, Kaige Wang, Tongsheng Chen, Junle Qu, and Hanben Niu, "Fast flexible multiphoton fluorescence lifetime imaging using acousto-optic deflector," Opt. Lett. 38, 1697-1699 (2013)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. H. J. Lin, P. Herman, and J. R. Lakowicz, Cytom. A 52, 77 (2003). [CrossRef]
  2. A. V. Agronskaia, L. Tertoolen, and H C Gerritsen, J. Phys. D 36, 1655 (2003). [CrossRef]
  3. K. Suhling, J. Siegel, D. Phillips, P. M. W. French, S. Lévêque-Fort, S. E. D. Webb, and D. M. Davis, Biophys. J. 83, 3589 (2002). [CrossRef]
  4. S. Kumar, C. Dunsby, P. A. De Beule, D. M. Owen, U. Anand, P. M. Lanigan, R. K. Benninger, D. M. Davis, M. A. Neil, P. Anand, C. Benham, A. Naylor, and P. M. French, Opt. Express 15, 12548 (2007). [CrossRef]
  5. W. Becker, J. Microsc. 247, 119 (2012). [CrossRef]
  6. V. Katsoulidou, A. Bergmann, and W. Becker, Proc. SPIE 6771, 67710B (2007). [CrossRef]
  7. D. McLoskey, D. Campbell, A. Allison, and G. Hungerford, Meas. Sci. Technol. 22, 067001 (2011). [CrossRef]
  8. F. A. Rosell, J. Opt. Soc. Am. A 50, 521 (1960). [CrossRef]
  9. N. D. Kirkpatrick, E. Chung, D. C. Cook, X. Han, G. Gruionu, S. Liao, L. L. Munn, T. P. Padera, D. Fukumura, and R. K. Jain, IntraVital 1, 60 (2012). [CrossRef]
  10. C W. Warger, S. A. Guerrera, Z. Eastman, and C. A. DiMarzio, Proc. SPIE 7184, 71840M (2009). [CrossRef]
  11. X. Lv, C. Zhan, H. Zhang, Q. Luo, W. R. Chen, and S. Zeng, Acta Optica Sinica 26, 1823 (2006).
  12. A. Bullen, S. S. Patel, and P. Saggau, Biophys. J. 73, 477 (1997). [CrossRef]
  13. V. Iyer, B. E. Losavio, and P. Saggau, J. Biomed. Opt. 8, 460 (2003). [CrossRef]
  14. R. Salomé, Y. Kremer, S. Dieudonné, J.-F. Léger, O. Krichevsky, C. Wyart, D. Chatenay, and L. Bourdieu, J. Neurosci. Methods 154, 161 (2006). [CrossRef]
  15. X. Lv, C. Zhan, S. Zeng, W. R. Chen, and Q. Luo, Rev. Sci. Instrum. 77, 046101 (2006). [CrossRef]
  16. P. A. Kirkby, K. M. N. Srinivas Nadella, and R. A. Silver, Opt. Express 18, 13720 (2010). [CrossRef]
  17. Y. Shao, W. Qin, H. Liu, J. Qu, X. Peng, H. Niu, and B. Z. Gao, Opt. Lett. 37, 2532 (2012). [CrossRef]
  18. M. A. Digman and E. Gratton, Microsc. Res. Tech. 72, 323 (2009). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1. Fig. 2. Fig. 3.
Fig. 4.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited