OSA's Digital Library

Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 38, Iss. 15 — Aug. 1, 2013
  • pp: 2800–2803

Calibration-free quantification of absolute oxygen saturation based on the dynamics of photoacoustic signals

Jun Xia, Amos Danielli, Yan Liu, Lidai Wang, Konstantin Maslov, and Lihong V. Wang  »View Author Affiliations

Optics Letters, Vol. 38, Issue 15, pp. 2800-2803 (2013)

View Full Text Article

Enhanced HTML    Acrobat PDF (578 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



Photoacoustic tomography (PAT) is a hybrid imaging technique that has broad preclinical and clinical applications. Based on the photoacoustic effect, PAT directly measures specific optical absorption, which is the product of the tissue-intrinsic optical absorption coefficient and the local optical fluence. Therefore, quantitative PAT, such as absolute oxygen saturation (sO2) quantification, requires knowledge of the local optical fluence, which can only be estimated through invasive measurements or sophisticated modeling of light transportation. In this Letter, we circumvent this requirement by taking advantage of the dynamics in sO2. The new method works when the sO2 transition can be simultaneously monitored with multiple wavelengths. For each wavelength, the ratio of photoacoustic amplitudes measured at different sO2 states is utilized. Using the ratio cancels the contribution from optical fluence and allows calibration-free quantification of absolute sO2. The new method was validated through both phantom and in vivo experiments.

© 2013 Optical Society of America

OCIS Codes
(100.6950) Image processing : Tomographic image processing
(110.5120) Imaging systems : Photoacoustic imaging
(170.1460) Medical optics and biotechnology : Blood gas monitoring
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(110.5125) Imaging systems : Photoacoustics

ToC Category:
Imaging Systems

Original Manuscript: May 17, 2013
Manuscript Accepted: June 29, 2013
Published: July 29, 2013

Virtual Issues
Vol. 8, Iss. 9 Virtual Journal for Biomedical Optics

Jun Xia, Amos Danielli, Yan Liu, Lidai Wang, Konstantin Maslov, and Lihong V. Wang, "Calibration-free quantification of absolute oxygen saturation based on the dynamics of photoacoustic signals," Opt. Lett. 38, 2800-2803 (2013)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. A. Cyrous, B. O’Neal, and W. D. Freeman, Expert Rev. Neurotherap. 12, 915 (2012). [CrossRef]
  2. M. Hockel and P. Vaupel, J. Natl. Cancer Inst. 93, 266 (2001). [CrossRef]
  3. D. Rischin, R. J. Hicks, R. Fisher, D. Binns, J. Corry, S. Porceddu, and L. J. Peters, J. Clin. Oncol. 24, 2098 (2006). [CrossRef]
  4. J. Xia, M. Chatni, K. Maslov, Z. Guo, K. Wang, M. Anastasio, and L. V. Wang, J. Biomed. Opt. 17, 050506 (2012). [CrossRef]
  5. M. R. Chatni, J. Xia, R. Sohn, K. Maslov, Z. Guo, Y. Zhang, K. Wang, Y. Xia, M. Anastasio, J. Arbeit, and L. V. Wang, J. Biomed. Opt. 17, 076012 (2012). [CrossRef]
  6. L. V. Wang and S. Hu, Science 335, 1458 (2012). [CrossRef]
  7. B. Cox, J. G. Laufer, S. R. Arridge, and P. C. Beard, J. Biomed. Opt. 17, 061202 (2012). [CrossRef]
  8. J. Laufer, D. Delpy, C. Elwell, and P. Beard, Phys. Med. Biol. 52, 141 (2007). [CrossRef]
  9. A. Q. Bauer, R. E. Nothdurft, T. N. Erpelding, L. V. Wang, and J. P. Culver, J. Biomed. Opt. 16, 096016 (2011). [CrossRef]
  10. Z. Guo, S. Hu, and L. V. Wang, Opt. Lett. 35, 2067 (2010). [CrossRef]
  11. L. Wang, S. L. Jacques, and L. Zheng, Comput. Methods Programs Biomed. 47, 131 (1995). [CrossRef]
  12. D. J. Faber, F. J. van der Meer, M. C. G. Aalders, D. M. de Bruin, and T. G. van Leeuwen, Proc. SPIE 5861, 58610W (2005). [CrossRef]
  13. S. Srinivasan, B. W. Pogue, S. Jiang, H. Dehghani, C. Kogel, S. Soho, J. J. Gibson, T. D. Tosteson, S. P. Poplack, and K. D. Paulsen, Proc. Natl. Acad. Sci. U.S.A. 100, 12349 (2003). [CrossRef]
  14. J. Gamelin, A. Maurudis, A. Aguirre, F. Huang, P. Guo, L. V. Wang, and Q. Zhu, Opt. Express 17, 10489 (2009). [CrossRef]
  15. J. Xia, Z. Guo, K. Maslov, A. Aguirre, Q. Zhu, C. Percival, and L. V. Wang, J. Biomed. Opt. 16, 090505 (2011). [CrossRef]
  16. M. H. Xu and L. H. V. Wang, Phys. Rev. E 71, 016706 (2005). [CrossRef]
  17. K. Maslov, H. F. Zhang, and L. V. Wang, Inverse Probl. 23, S113 (2007). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1. Fig. 2. Fig. 3.
Fig. 4.

Supplementary Material

» Media 1: MOV (1809 KB)     
» Media 2: MOV (5912 KB)     
» Media 3: MOV (14126 KB)     

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited