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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 38, Iss. 24 — Dec. 15, 2013
  • pp: 5280–5283

Comparison of line-scanned and point-scanned dual-axis confocal microscope performance

D. Wang, Y. Chen, Y. Wang, and J. T. C. Liu  »View Author Affiliations

Optics Letters, Vol. 38, Issue 24, pp. 5280-5283 (2013)

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The point-scanned dual-axis confocal (PS-DAC) microscope has been shown to exhibit superior capability to reject out-of-focus and multiply scattered light in comparison to its conventional single-axis counterpart. However, the slow frame rate (typically <5Hz) resulting from point-by-point data collection makes these systems vulnerable to motion artifacts. While video-rate point-scanned confocal microscopy is possible, a line-scanned dual-axis confocal (LS-DAC) microscope provides a simpler means of achieving high-speed imaging through line-by-line data collection, but sacrifices contrast due to loss of confocality along one dimension. Here we evaluate the performance trade-offs between an LS-DAC and PS-DAC microscope with identical spatial resolutions. Characterization experiments of the LS-DAC and PS-DAC microscopes with tissue phantoms, in reflectance mode, are shown to match results from Monte Carlo scattering simulations of the systems. Fluorescence images of mouse brain vasculature, obtained using resolution-matched LS-DAC and PS-DAC microscopes, demonstrate the comparable performance of LS-DAC and PS-DAC microscopy at shallow depths.

© 2013 Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5810) Medical optics and biotechnology : Scanning microscopy
(220.4830) Optical design and fabrication : Systems design

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: September 30, 2013
Revised Manuscript: November 6, 2013
Manuscript Accepted: November 7, 2013
Published: December 4, 2013

Virtual Issues
Vol. 9, Iss. 2 Virtual Journal for Biomedical Optics

D. Wang, Y. Chen, Y. Wang, and J. T. C. Liu, "Comparison of line-scanned and point-scanned dual-axis confocal microscope performance," Opt. Lett. 38, 5280-5283 (2013)

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  1. J. M. Jabbour, M. A. Saldua, J. N. Bixler, and K. C. Maitland, Annals Biomed. Eng. 40, 378 (2012). [CrossRef]
  2. J. T. Liu, N. O. Loewke, M. J. Mandella, R. M. Levenson, J. M. Crawford, and C. H. Contag, Analyt. Cell. Pathol. 34, 81 (2011).
  3. T. Wilson, Confocal Microscopy (Academic, 1990).
  4. J. B. Pawley, Handbook of Biological Confocal Microscopy, Rev. ed. (Plenum, 1990).
  5. C. Pitris, B. Bouma, M. Shiskov, and G. Tearney, Opt. Express 11, 120 (2003). [CrossRef]
  6. C. MacAulay, P. Lane, and R. Richards-Kortum, Gastrointest. Endosc. Clin. North America 14, 595, xi (2004). [CrossRef]
  7. R. Kiesslich, J. Burg, M. Vieth, J. Gnaendiger, M. Enders, P. Delaney, A. Polglase, W. McLaren, D. Janell, S. Thomas, B. Nafe, P. R. Galle, and M. F. Neurath, Gastroenterology 127, 706 (2004). [CrossRef]
  8. F. Jean, G. Bourg-Heckly, and B. Viellerobe, Opt. Express 15, 4008 (2007). [CrossRef]
  9. J. T. Liu, M. J. Mandella, N. O. Loewke, H. Haeberle, H. Ra, W. Piyawattanametha, O. Solgaard, G. S. Kino, and C. H. Contag, J. Biomed. Opt. 15, 026029 (2010). [CrossRef]
  10. H. Makhlouf, A. F. Gmitro, A. A. Tanbakuchi, J. A. Udovich, and A. R. Rouse, J. Biomed. Opt. 13, 044016 (2008). [CrossRef]
  11. D. L. Dickensheets and G. S. Kino, Opt. Lett. 21, 764 (1996). [CrossRef]
  12. G. J. Tearney, R. H. Webb, and B. E. Bouma, Opt. Lett. 23, 1152 (1998). [CrossRef]
  13. Y. S. Sabharwal, A. R. Rouse, L. Donaldson, M. F. Hopkins, and A. F. Gmitro, Appl. Opt. 38, 7133 (1999). [CrossRef]
  14. J. T. Liu, M. J. Mandella, J. M. Crawford, C. H. Contag, T. D. Wang, and G. S. Kino, J. Biomed. Opt. 13, 034020 (2008). [CrossRef]
  15. T. D. Wang, C. H. Contag, M. J. Mandella, N. Y. Chan, and G. S. Kino, J. Biomed. Opt. 9, 735 (2004). [CrossRef]
  16. J. T. Liu, M. J. Mandella, S. Friedland, R. Soetikno, J. M. Crawford, C. H. Contag, G. S. Kino, and T. D. Wang, J. Biomed. Opt. 11, 054019 (2006). [CrossRef]
  17. Y. Chen, D. Wang, and J. T. Liu, Opt. Lett. 37, 4495 (2012). [CrossRef]
  18. Y. Chen and J. T. Liu, J. Biomed. Opt. 18, 066006 (2013). [CrossRef]
  19. W. Piyawattanametha, H. Ra, Z. Qiu, S. Friedland, J. T. Liu, K. Loewke, G. S. Kino, O. Solgaard, T. D. Wang, M. J. Mandella, and C. H. Contag, J. Biomed. Opt. 17, 021102 (2012). [CrossRef]
  20. H. Ra, W. Piyawattanametha, E. Gonzalez-Gonzalez, M. J. Mandella, G. S. Kino, O. Solgaard, D. Leake, R. L. Kaspar, A. Oro, and C. H. Contag, J. Invest. Dermatol. 131, 1061 (2011).
  21. P. J. Dwyer, C. A. DiMarzio, and M. Rajadhyaksha, Appl. Opt. 46, 1843 (2007). [CrossRef]
  22. B. Larson, S. Abeytunge, and M. Rajadhyaksha, Biomed. Opt. Express 2, 2055 (2011). [CrossRef]
  23. M. Rajadhyaksha, R. R. Anderson, and R. H. Webb, Appl. Opt. 38, 2105 (1999). [CrossRef]
  24. J. T. Liu, M. J. Mandella, H. Ra, L. K. Wong, O. Solgaard, G. S. Kino, W. Piyawattanametha, C. H. Contag, and T. D. Wang, Opt. Lett. 32, 256 (2007). [CrossRef]
  25. C. J. R. Sheppard and X. Q. Mao, J. Mod. Opt. 35, 1169 (1988). [CrossRef]
  26. T. Wilson and S. J. Hewlett, J. Microsc. 160, 115 (1990). [CrossRef]
  27. S. M. Mansfield, W. R. Studenmund, G. S. Kino, and K. Osato, Opt. Lett. 18, 305 (1993). [CrossRef]
  28. B. Chance, P. van der Zee, M. Essenpreis, D. T. Delpy, and R. R. Alfano, Proc. SPIE 1888, 454 (1993).
  29. D. Wang, Y. Chen, and J. T. Liu, Biomed. Opt. Express 3, 3153 (2012). [CrossRef]
  30. C. Glazowski and M. Rajadhyaksha, J. Biomed. Opt. 17, 085001 (2012). [CrossRef]

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