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Optics Letters

Optics Letters


  • Editor: Xi-Cheng Zhang
  • Vol. 39, Iss. 11 — Jun. 1, 2014
  • pp: 3352–3355

Simultaneous measurement of position and color of single fluorescent emitters using diffractive optics

Jordi Broeken, Bernd Rieger, and Sjoerd Stallinga  »View Author Affiliations

Optics Letters, Vol. 39, Issue 11, pp. 3352-3355 (2014)

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We propose a method for simultaneously measuring the position and emission color of single fluorescent emitters based on the use of a large pitch diffraction grating in the emission light path. The grating produces satellite spots adjacent to the main spot; the relative distance between the spots is a measure for the emission wavelength. We present proof-of-principle experiments on beads and mixtures of quantum dots using a spatial light modulator for making a programmable diffraction grating. A wavelength precision of around 10 nm can be achieved for 1000 signal photons and practical background levels, while maintaining a localization precision of around 10 nm.

© 2014 Optical Society of America

OCIS Codes
(050.1950) Diffraction and gratings : Diffraction gratings
(100.6640) Image processing : Superresolution
(180.2520) Microscopy : Fluorescence microscopy
(230.6120) Optical devices : Spatial light modulators
(300.0300) Spectroscopy : Spectroscopy

ToC Category:

Original Manuscript: December 20, 2013
Revised Manuscript: April 15, 2014
Manuscript Accepted: April 27, 2014
Published: May 30, 2014

Jordi Broeken, Bernd Rieger, and Sjoerd Stallinga, "Simultaneous measurement of position and color of single fluorescent emitters using diffractive optics," Opt. Lett. 39, 3352-3355 (2014)

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