OSA's Digital Library

Optics Letters

Optics Letters


  • Editor: Xi-Cheng Zhang
  • Vol. 39, Iss. 12 — Jun. 15, 2014
  • pp: 3468–3471

Quantitative phase imaging using a deep UV LED source

Alok Kumar Singh, Ahmad Faridian, Peng Gao, Giancarlo Pedrini, and Wolfgang Osten  »View Author Affiliations

Optics Letters, Vol. 39, Issue 12, pp. 3468-3471 (2014)

View Full Text Article

Enhanced HTML    Acrobat PDF (431 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We propose a method for high resolution phase imaging of biological and non-biological samples using an incoherent deep ultraviolet (DUV) LED source. The diffraction pattern of the object wave is recorded at different axial planes and the phase is retrieved by propagation of the angular spectrum. To maintain enough light intensity, we avoided using a pinhole or spectral filter for increasing the coherence of the DUV LED source. This makes the setup very simple and cost effective. The short wavelength (285 nm) of the DUV light, tuned to the absorption peak of the biological samples, allows simultaneously high resolution and high contrast images. The experimental results are presented to verify this principle.

© 2014 Optical Society of America

OCIS Codes
(030.1640) Coherence and statistical optics : Coherence
(100.5070) Image processing : Phase retrieval
(110.4980) Imaging systems : Partial coherence in imaging
(090.1995) Holography : Digital holography

ToC Category:
Imaging Systems

Original Manuscript: February 7, 2014
Revised Manuscript: May 7, 2014
Manuscript Accepted: May 8, 2014
Published: June 5, 2014

Virtual Issues
Vol. 9, Iss. 8 Virtual Journal for Biomedical Optics

Alok Kumar Singh, Ahmad Faridian, Peng Gao, Giancarlo Pedrini, and Wolfgang Osten, "Quantitative phase imaging using a deep UV LED source," Opt. Lett. 39, 3468-3471 (2014)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. M. Wurm, F. Pilarski, and B. Bodermann, Rev. Sci. Instrum. 81, 023701 (2010). [CrossRef]
  2. X. Niu, N. H. Jakatdar, J. Bao, C. J. Spanos, and S. K. Yedur, Proc. SPIE 3677, 159 (1999). [CrossRef]
  3. W. Osten, V. F. Paz, K. Frenner, T. Schuster, and H. Bloess, Proc. AIP 1173, 371 (2009).
  4. B. Bodermann, E. Buhr, G. Ehret, F. Scholze, and M. Wurm, Proc. SPIE 7155, 71550V (2008). [CrossRef]
  5. B. J. Zeskind, C. D. Jordan, W. Timp, L. Trapani, G. Waller, V. Horodincu, D. J. Ehrlich, and P. Matsudaira, Nat. Methods 4, 567 (2007). [CrossRef]
  6. A. Faridian, D. Hopp, G. Pedrini, U. Eigenthaler, M. Hirscher, and W. Osten, Opt. Express 18, 14159 (2010). [CrossRef]
  7. A. Faridian, D. Hopp, G. Pedrini, and W. Osten, Proc. SPIE 7718, 771803 (2010). [CrossRef]
  8. U. Gopinathan, G. Pedrini, B. Javidi, and W. Osten, J. Display Technol. 6, 479 (2010). [CrossRef]
  9. A. Anand, A. Faridian, V. Chhaniwal, G. Pedrini, W. Osten, and B. Javidi, Opt. Lett. 36, 4362 (2011). [CrossRef]
  10. R. Guo, B. Yao, P. Gao, J. Min, M. Zhou, J. Han, X. Yu, X. Yu, M. Lie, S. Yan, Y. Yang, D. Dan, and T. Ye, Appl. Opt. 52, 8233 (2013). [CrossRef]
  11. S. Stürwald, B. Kemper, C. Remmersmann, P. Langehanenberg, and G. von Bally, Proc. SPIE 6995, 699507 (2008). [CrossRef]
  12. F. Dubois and C. Yourassowsky, Opt. Lett. 37, 2190 (2012). [CrossRef]
  13. F. Dubois, L. Joannes, and J. C. Legros, Appl. Opt. 38, 7085 (1999). [CrossRef]
  14. B. Kemper, S. Stürwald, C. Remmersmann, P. Langehanenberg, and G. von Bally, Opt. Lasers Eng. 46, 499 (2008). [CrossRef]
  15. G. Pedrini, W. Osten, and Y. Zhang, Opt. Lett. 30, 833 (2005). [CrossRef]
  16. P. Almoro, G. Pedrini, and W. Osten, Appl. Opt. 45, 8596 (2006). [CrossRef]
  17. F. Zhang, G. Pedrini, and W. Osten, Phys. Rev. A 75, 043805 (2007). [CrossRef]
  18. A. Mazine and K. Heggarty, Appl. Opt. 50, 2679 (2011). [CrossRef]
  19. L. J. Allen and M. P. Oxley, Opt. Commun. 199, 65 (2001). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1. Fig. 2. Fig. 3.
Fig. 4.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited