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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 2 — Feb. 1, 2012

In vivo confocal imaging of fast intrinsic optical signals correlated with frog retinal activation

Qiu-Xiang Zhang, Rong-Wen Lu, Yang-Guo Li, and Xin-Cheng Yao  »View Author Affiliations

Optics Letters, Vol. 36, Issue 23, pp. 4692-4694 (2011)

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Using freshly isolated animal retinas, we have conducted a series of experiments to test fast intrinsic optical signals (IOSs) that have time courses comparable to electrophysiological kinetics. In this Letter, we demonstrate the feasibility of in vivo imaging of fast IOSs in intact frogs. A rapid line-scan confocal ophthalmoscope was constructed to achieve high-speed IOS recording. By rejecting out-of-focus background light, the line-scan confocal imager provided the resolution to differentiate individual photoreceptors in vivo. Rapid confocal imaging disclosed robust IOSs with time courses comparable to retinal electroretinogram kinetics. High-resolution IOS images revealed both positive (increasing) and negative (decreasing) light responses, with subcellular complexity.

© 2011 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.2655) Medical optics and biotechnology : Functional monitoring and imaging
(170.5755) Medical optics and biotechnology : Retina scanning

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: September 20, 2011
Revised Manuscript: October 27, 2011
Manuscript Accepted: October 28, 2011
Published: December 1, 2011

Virtual Issues
Vol. 7, Iss. 2 Virtual Journal for Biomedical Optics

Qiu-Xiang Zhang, Rong-Wen Lu, Yang-Guo Li, and Xin-Cheng Yao, "In vivo confocal imaging of fast intrinsic optical signals correlated with frog retinal activation," Opt. Lett. 36, 4692-4694 (2011)

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