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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: Joseph N. Mait
  • Vol. 50, Iss. 34 — Dec. 1, 2011
  • pp: H253–H264

Partially coherent lensfree tomographic microscopy [Invited]

Serhan O. Isikman, Waheb Bishara, and Aydogan Ozcan  »View Author Affiliations


Applied Optics, Vol. 50, Issue 34, pp. H253-H264 (2011)
http://dx.doi.org/10.1364/AO.50.00H253


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Abstract

Optical sectioning of biological specimens provides detailed volumetric information regarding their internal structure. To provide a complementary approach to existing three-dimensional (3D) microscopy modalities, we have recently demonstrated lensfree optical tomography that offers high-throughput imaging within a compact and simple platform. In this approach, in-line holograms of objects at different angles of partially coherent illumination are recorded using a digital sensor-array, which enables computing pixel super-resolved tomographic images of the specimen. This imaging modality, which forms the focus of this review, offers micrometer-scale 3D resolution over large imaging volumes of, for example, 1015mm3, and can be assembled in light weight and compact architectures. Therefore, lensfree optical tomography might be particularly useful for lab-on-a-chip applications as well as for microscopy needs in resource-limited settings.

© 2011 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(110.1758) Imaging systems : Computational imaging
(090.1995) Holography : Digital holography
(110.6955) Imaging systems : Tomographic imaging

ToC Category:
Invited ISP Papers

History
Original Manuscript: August 1, 2011
Revised Manuscript: October 11, 2011
Manuscript Accepted: October 11, 2011
Published: December 5, 2011

Virtual Issues
Vol. 7, Iss. 2 Virtual Journal for Biomedical Optics
Digital Holography and 3D Imaging 2011 (2011) Applied Optics

Citation
Serhan O. Isikman, Waheb Bishara, and Aydogan Ozcan, "Partially coherent lensfree tomographic microscopy [Invited]," Appl. Opt. 50, H253-H264 (2011)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-50-34-H253

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